tzanck smear method - HEALTHY
The classic laboratory methods for the detection and differentiation of HSV-1/2 and VZV, including Tzanck smear testing, viral culture, DFA testing and serologic testing, have been well documented in ... The Tzanck smear assay is a cytological, morphological examination method that was used for many years for the diagnosis of various skin and mucocutaneous infections with HSV and VZV. The classic laboratory methods for the detection and differentiation of HSV-1/2 and VZV, including Tzanck smear testing, viral culture, DFA testing and serologic testing, have been well documented ...
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The quickest method of diagnosis is the time-honored Tzanck preparation. A #15 surgical blade should be used to open the top of a vesicle, scraping the underside of the vesicle as well as the base. The diagnosis is made clinically, but may be confirmed by a Tzanck smear (looking for multinucleated giant cells), a fluorescent antibody smear or culture of a vesicular lesion. We performed a Tzanck preparation, which showed multinucleated giant keratinocytes with nuclear molding and margination (Appendix Figure).
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A direct fluorescent antibody (DFA) test was positive for ... Therapy should be instituted without delay, with high suspicion or positive Tzanck preparation. Acyclovir is the usual treatment, and for severe disease in immunocompromised patients, is dosed at ...